Point light sources showing the highest brightness density, homogeneity and flexibility


The latest innovation of GATTAquant is a new type of fluorescent beads, ideal for determining the point spread function in optical microscopy. gattabeads are the perfect choice for STED microscopy and other fluorescence techniques requiring small but bright point sources of light.

Copyright by Leica Microsystems

Application

Where can you apply the new gattabeads to?


  • Setup calibration
  • 2D STED measurements
  • 3D STED PSF distillation and deconvolution
  • Tracking and drift-correction
3D stack of GATTA-Beads R measured at VUmc, Amsterdam, on a Leica TCS SP8 STED 3X. 3D PSF distillation and rendering by Scientific Volume Imaging.

Advantages

Get an overview of the unique properties

gattabeads exhibit the highest brightness density in the world. With an average diameter of 23 nm they are brighter than other commercial beads having a diameter of 40 nm.

Left image: Intensity plot from confocal scans of GATTA-Beads R (dark grey) with a diameter of d = 23 nm compared to Dark-Red FluoSpheres® (light grey) with d = 40 nm. Fitted mean values: 15710 a.u. (GATTA-Beads), 12750 a.u. (FluoSpheres).

Right image: STED image of GATTA-Beads R measured on a Leica TCS SP8 STED 3X system.

DNA nanotechnology allows a highly parallelized production of nano beads with negligible size distribution. Together with the controlled patterning of dye molecules and the consequently minimized self-quenching, gattabeads show a superior narrow brightness distribution and unbiased fluorescence lifetimes.

Left image: Intensity plot from confocal scans of GATTA-Beads R (dark grey) with a diameter of d = 23 nm compared to Dark-Red FluoSpheres® (light grey) with d = 40 nm. Standard deviations: ± 1312 a.u. (GATTA-Beads), ± 5552 a.u. (FluoSpheres).

Right image: Lifetimes of GATTA-Beads R (dark grey) and Dark-Red FluoSpheres® (light grey). τ = 3.3 ± 0.1 ns (GATTA-Beads), τ = 2.2 ± 0.2 ns (FluoSpheres).

gattabeads can be labeled with all kinds of available fluorophores. For instance this allows you to calibrate your STED system with exactly the same dye you are using in your experiment – no change in spectral and photo physical properties will occur. Further gattabeads enable the attachment of various chemical groups, like biotin supporting your surface immobilization.

Bead-Series

Explore our product portfolio

GATTAquant provides you with the most stable and popular dyes in fluorescence microscopy. Browse below in our best choice of fluorophores regarding brightness and photo stability. Beside that various color alternatives are at hand and allow you the flexibility you need. On request also other fluorophores or modifications are feasible.

“… this represents the first accurate measurement of the three-dimensional PSF of a STED microscope with fluorescent beads, having a diameter smaller than 40 nm.”


In cooperation with Leica Microsystems, Germany, and Scientific Volume Imaging, The Netherlands, gattabeads were used to image and determine the three-dimensional point spread function under STED conditions. The z stacks enabled the precise distillation of the PSF based on real data and its utilization for deconvolution.

"… the brightest and most stable beads that I have
seen so far in the size range of 20–50 nm."


Ulf Schwarz
Application Manager, Leica Microsystems, Mannheim, Germany

Technology

The science behind the beads

Just as much as the nanorulers from GATTAquant, gattabeads are based on the DNA origami technique. This technique allows the precise placement of fluorophores on predefined patterns on the nanoscale. In case of the gattabeads this technique enables the controlled arrangement of fluorophores in closest proximity without introducing undesired photo physical interactions. According to this kind of controlled dye patterning the total brightness of the beads scales approximately linearly with the intensity of the single fluorophores, leading to the highest brightness density shown so far.


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